The Amersham™ HCPQuant™ CHO Supernatant Kit is a kit for the sensitive detection of host cell proteins in CHO cell lines. The Kit meets industry standards for specificity and sensitivity. It is specifically designed for standard to high throughput applications and can be easily adapted for automation. Key feature of this kit is the unmatched broad CHO-HCP contaminant coverage. In quality control applications, subject to thorough validation and qualification by each laboratory, the Amersham™ HCPQuant™ CHO Supernatant Kit may also be useful for lot release testing.
The Amersham™ HCPQuant™ CHO Supernatant Kit is a generic HCP kit. Generic assays are frequently used early in biological development when the process is still poorly defined. Biologic manufacturers often migrate to a process-specific HCP assay in later stages of development. The generic HCP assay has utility supporting early process development workflow. The CHO HCP ELISA kit uses polyclonal antibodies generated against CHO-K1 cell lysates that were derived from cells grown in chemically-defined media. These antibodies react strongly with CHO-K1 cell lysates and currently show the market-wide highest sensitivity for cells harvested at 60-85 % viability.
Features of Amersham™ HCPQuant™ CHO Supernatant Kit
In short:
- Generic ELISA kit for precise quantitation of HCP contaminants
- Market-wide broadest CHO-HCP contaminant coverage
- Low variability, high reproducibility
- very high sensitivity: detect as little as 2 ng/mL
- Low lot-to-lot variation
- Rapid time to result, easy to use
The antibodies in the kit were specially developed for the detection of HCP impurities and for the measurement of analytes from crude cellular harvests, cell culture lysates and process intermediates. The Amersham™ HCPQuant™ CHO Supernatant Kit uses purified antibodies generated against a CHO-K1 cell lysate from chemically defined media. These antibodies react strongly with CHO-K1 cell lysates and show the highest sensitivity for cells harvested at 60-85% viability. The detection and HCP quantification of samples obtained from cell culture fluid (CCF) will vary.
Coverage Comparison with a Competitor’s CHO HCP Antibody
Visualization by a high sensitivity in-gel protein stain. CHO-HCP proteins were detected by anti-CHO-HCP antibody, used in the kit, and a competitor’s generic anti-CHO-HCP antibody via 2D Western Blot using HRP detection.
- On the right: competitor’s CHO-HCP antibody
- Blue = Detection by antibody only
- Green = Detection by stain + antibody
- Red = Detection by stain only.
Coverage Comparison: on the left the CHO-HCP polyclonal antibody used in CHO HCP ELISA Kit. (click image to enlarge)
Comparative Results
| Coverage Analysis | Total Coverage | Q1 | Q2 | Q3 | Q4 |
| Commercial CHO-HCP Antibody | 79% | 96% | 92% | 67% | 59% |
| HCPQuant™ CHO Supernatant Kit Antibody | 89% | 99% | 91% | 92% | 76% |
Commercial CHO HCP antibody
The sensitivity is robust for proteins in the Q1 and Q2 quadrant. Coverage of the difficult to detect proteins coverage in quadrants q3 and q4 is between 50% and 70%. Total coverage of the competitor’s CHO-HCP antibody is 79%.
CHO HCP antibody used in the kit
The sensitivity of the antibody consistent for proteins in all 4 quadrants. Coverage of the difficult to detect proteins coverage in quadrants q3 and q4 is between 70% and 90%. Total coverage of the antibody across all quadrants is 89%.
Amersham™ HCPQuant™ CHO Supernatant Kit contains:
- CHO-HCP antibody coated 96-well strip plate
- Plate Sealer
- CHO-HCP standard
- CHO-HCP detection antibody
- HCP Sample Buffer
- HCP Wash Buffer
- HCP TMB Buffer
- HCP Stop Buffer
- protocol
More information required?
If you would like to have more information about the Amersham™ HCPQuant™ CHO Supernatant Kit, be free to contact us via email or phone or customer support team.