Below you will find an overview of HCP antibodies offered by antibodies-online.com and Rockland Immunochemicals Inc. Currently, products for the reactivities/species E. coli, CHO, and Human are available. Further information can be obtained by clicking on the links in the table. The HCP antibody you need is not on the list? Get in touch and discuss custom HCP solutions with our expert team.
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| Product | Product ID | Reactivity | Immunogen | Applications |
|---|---|---|---|---|
| Rabbit Anti-E. coli HCP Antibody | ABIN1607604 100-401-J07 | E. coli | Blend of antibodies immunized with high molecular weight (HMW) + low molecular weight (LMW) proteins from E. coli | WB, 2D-WB, ELISA |
| Rabbit Anti-E.coli Combined HCP Antibody | 100-401-M61 200-401-M61 | E. coli | High molecular weight (HMW) + low molecular weight (LMW) proteins from E. coli | WB, 2D-WB, ELISA |
| Rabbit Anti-CHO HCP Antibody | ABIN1113181 | CHO, Chinese hamster | Whole cell lysate of CHO-K1 cells | WB |
| Rabbit Anti-E. coli HCP Antibody | ABIN1113183 | E. coli | Lysate of E coli BL21 cells | WB |
| Rabbit Anti-Human HCP Antibody | ABIN1113182 | Human | Lysate of HEK293 cells | WB |
| Rabbit Anti-E. coli HMW HCP Antibody | 100-401-J05 200-401-J05 | E. coli | High molecular weight (HMW) proteins from E. coli | WB, 2D-WB, ELISA |
| Rabbit Anti-E. coli LMW HCP Antibody | ABIN5596745 100-401-J06 200-401-J06 | E. coli | Low molecular weight (LMW) proteins from E. coli | WB, 2D-WB, ELISA |
Validation data of the HCP antibodies above
Rabbit anti-E. coli HCP antibody, ABIN1607604 Order information
This anti-HCP antibody recognizes high and low molecular weight protein fractions from E.coli. Available validation data for Western Blot, 2D-Western Blot and ELISA.
2D Western Blot of anti-E.coli HCP antibody. Load: 35 µg Total HCP. pH: 5-8. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: ABIN925618 for 1 hour at RT.
Western Blot of Rabbit anti-HCP antibody. Lane 1: Molecular Weight. Lane 2: Total HCP. Load: 10µg per lane. Primary antibody: Rabbit anti-HCP cocktail at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: ABIN925618 for 1 hour at RT.
ELISA of Rabbit anti-Host Cell Protein antibody. Antigen: BSA conjugates of HCP. Coating amount: 1 µg per well titrated 1:3 in 1XPBS. Primary antibody: Anti-HCP antibody at 100 ng/well. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000. Substrate: TMB for 30min.
Rabbit anti-CHO HCP antibody, ABIN1113181 Order information
Antibody for the detection of HCP in CHO cells (chinese hamster ovarian cells). Available validation data for Western Blot.
WB image for anti-Host Cell Proteins (HCP) antibody (ABIN1113181). Western-blotting of CHO cell Lysate
Rabbit anti-E. coli HCP antibody, ABIN1113183 Order information
Antibody for the detection of Host Cell Proteins. Expression system E.coli (BL21). Available validation data for Western Blotting.
WB image for anti-Host Cell Proteins (HCP) antibody (ABIN1113183). Western-blotting of E coli cell Lysate
Rabbit anti-Human HCP antibody, ABIN1113182 Order information
Antibody for the detection of Host Cell Proteins. Expression system HEK293T cells. Available validation data for Western Blot.
WB image for anti-Host Cell Proteins (HCP) antibody (ABIN1113182). Western-blotting of HEK cell Lysate.
Rabbit anti-E. coli HMW HCP antibody, ABIN5596742 Order information
This anti-HMW HCP Antibody recognizes high molecular weight protein fractions from E.coli. HMW Host Cell Protein A purified antibody is directed against high molecular weight E. coli Host Cell Proteins. Available validation data for 2D-Western Blot and ELISA.
2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 µg HMW HCP. Primary antibody: Rabbit anti-HMW-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: ABIN925618 for 1 hour at RT.
ELISA of Rabbit Anti-E.coli High Molecular Weight Host Cell Protein Antibody. Antigen: Total HCP protein. Coating amount: 0.1 µg per well. Primary antibody: E.coli HMW HCP antibody at 10 µg/mL. Dilution series: 2-fold. Mid-point concentration: 5 ng/mL LMW HCP antibody. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000. Substrate: TMB .
Rabbit anti-E. coli LMW HCP antibody, ABIN5596745 Order information
This anti-LMW E. coli HCP Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E. coli. Immunogen Type: Native Protein. Available validation data for 2D-WB and ELISA.
2D Western Blot of anti-E.coli Low Molecular Weight Host Cell Protein antibody. Load: 35 µg LMW HCP. Primary antibody: Rabbit anti-LMW-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: ABIN925618 for 1 hour at RT.
ELISA of Rabbit anti-Low Molecular Weight Host Cell Protein antibody. Antigen: BSA conjugates of LMW HCP. Coating amount: 1 µg per well titrated 1:3 in 1XPBS. Primary antibody: Anti-LMW-HCP antibody at 100 ng/well. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000. Substrate: TMB for 30min.
Secondary antibodies are commonly used in HCP projects in combination with primary antibodies that recognize specific HCPs. Secondary antibodies can be conjugated to various detection molecules, such as fluorescent dyes or enzymes, which can amplify the signal and improve the sensitivity of the assay.